Coding

Part:BBa_K2387002:Design

Designed by: Bart Scholten   Group: iGEM17_Wageningen_UR   (2017-10-14)


Transmembrane signal transduction protein CpxA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Deleted illegal EcoR1 site at amino acids Glu217 and Phe218(GAATTC --> GAGTTT) using silent mutations


Source

E. coli K12 genomic DNA

References